Detection of Isoniazid-Resistant Clinical isolates of Mycobacterium tuberculosis from India using Ser315Thr marker by Comparison of molecular methods

detection of isoniazid-resistant clinical isolates of mycobacterium tuberculosis from india using ser315thr marker by comparison of molecular methods

in this study, substitution at codon ser315 of katg gene, a reliable marker for isoniazid (inh) resistance was analyzed and compared by three molecular methods such as dna sequencing, polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) and pcr-single strand conformation polymorphism (pcr-sscp) in 105 phenotypically resistant isolates obtained from various parts of indi...

Virulence in isoniazid-resistant clinical isolates of Mycobacterium tuberculosis from south India

Isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. INH-resistant mutants were shown to contain defective katG gene. Classical studies showed that INH-resistant south Indian isolates have lower virulence in guinea pigs and higher susceptibility to H2O2. It is of interest to assess the virulence in south India...

virulence in isoniazid-resistant clinical isolates of mycobacterium tuberculosis from south india

isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. inh-resistant mutants were shown to contain defective katg gene. classical studies showed that inh-resistant south indian isolates have lower virulence in guinea pigs and higher susceptibility to h2o2. it is of interest to assess the virulence in south india...

Virulence in isoniazid-resistant clinical isolates of Mycobacterium tuberculosis from south India

Molecular characterisation of isoniazid resistant clinical isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan.

OBJECTIVE To investigate the frequency of mutations in catalase-peroxidase and inhibin alpha genes in clinical isolates of isoniazid resistant Mycobacterium tuberculosis strains. METHODS The study was conducted at Provincial Tuberculosis Reference Laboratory, Peshawar, Pakistan, from April 2015 to March 2016, and comprised sputum specimens obtained from patients of different ages. All the iso...

Detection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates

Abstract Background and Objective: Increasing prevalence of methicillin resistant Staphylococcus aureus strains (MRSA) with their multidrug resistance potential causes difficulties in the treatment of infections due to these bacteria. Hence, the detection and determination of the frequency of MRSA strains via phenotypical and molecular methods is necessary in different parts of the county. ...